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96992
Sigma-Aldrich

CELL COUNTING KIT - 8 CELL COUNTING KIT - 8

Nacres: NA.32
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Properties

Properties
Description CELL COUNTING KIT - 8 CELL COUNTING KIT - 8
Import License Not required
Check Obsolete No
Temperature Control 10
Shipping Mode DAS
Business Unit Diagnostics & Regulated Materials

Description

Application

Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. CCK-8 solution is added directly to the cells, no pre-mixing of components is required. WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.

SAFETY INFORMATION

GHS07,GHS08

Signal Word

Warning

H315 - H319 - H341 - H351

P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

RIDADRRIDADR

WGK Germany

WGK 2

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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