Description
- Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria.
- It has been used in the cytotoxicity assay buffer.
- It has been used for the induction of protein overload nephropathy in rats.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.
Often referred to as Cohn fraction V
this product is prepared by a modified method of the Cohn cold ethanol fractionation method.