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G1881
Sigma-Aldrich

A-GLYCEROPHOSPHATE DEHYDR OGENASE- TRIOSE

Nacres: -Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle
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Properties

Properties
biological source rabbit muscle
Description A-GLYCEROPHOSPHATE DEHYDR OGENASE- TRIOSE
form ammonium sulfate suspension
gdh activity 75-200 
gel capacity units/mg protein (biuret)
Import License Not required
Check Obsolete No
Temperature Control 10
Shipping Mode DAS
Business Unit Biology

Description

General description

Application

α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle has been used in spectrophotometric aldolase assay and fructose 2,6 bisphosphate assay. α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay.

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase (α-GPDH) has the ability to produce reactive oxygen species (ROS), that is induced by Ca2+. α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate.

Packaging

(Sold on the basis of α-GDH units.)

Unit Definition

(α-GDH) One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C. (TPI) One unit will convert 1.0 μmole of D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Mixed crystalline suspension in 3.2 M (NH4)2SO4, with 0.05 g/L EDTA, pH 6.0

Warning

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 2

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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