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Library preparation is the first step of next generation sequencing. Before DNA or RNA samples can be sequenced, nucleic acids must be isolated, fragmented, end-repaired, and covalently linked to adapters using ligation or tagmentation methods. The complexity of a well-prepared NGS library should fully and accurately reflect the complexity of the sample, but will also reflect any biases introduced during library preparation. A key goal in preparing a DNA or RNA library for next generation sequencing is to maximize complexity while reducing PCR or other amplification-introduced biases, as the quality of the resulting library can strongly impact NGS results.
We offer a portfolio of NGS library preparation tools designed to simplify workflows and facilitate the preparation of high quality DNA or RNA libraries that accurately represent sample complexity while reducing bias for whole genome sequencing (WGS), whole transcriptome analysis (WTA), total RNA sequencing, and miRNA and small RNA sequencing applications.
Products
Product Number | Product Description | Pricing |
---|---|---|
AMPD1 | DEOXYRIBONUCLEASE I, KIT AMPLIFICATIONGR | Expand |
PCR9604 | GENELUTE 96 WELL PCR CLEA N-UP KIT | Expand |
NA1020 | GENELUTE PCR CLEAN-UP KIT | Expand |
KMM-101NV | KOD One(TM) PCR Master Mi x, Ready-to- | Expand |
KMM-201NV | KOD One(TM)PCR Master Mix -BLUE-, Read & | Expand |
L3024 | LIPOPOLYSACCHARIDES FROM ESCHERICHIA COL | Expand |
WGA4 | SINGLE CELL WGA KIT SINGLE CELL WGA KIT | Expand |
S9430 | SYBR GREEN I | Expand |
W4502 | WATER MOLECULAR BIOLOGY R WATER MOLECULAR BIOLOGY R | Expand |