NRK-52E has been used to study the effects of altered selenium supply on the expression of endogenous thioredoxin reductase (TrxR).
Cloned from the same mixed culture of normal rat kidney cells as NRK-49F, but has epithelial-like morphology. It has distinct growth and transforming characteristics distinct from NRK-49F.
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).
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Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Remove all traces of trypsin from cultures during sub-culturing i.e. spin cells or neutralise with serum.