google-site-verification=e-m9AOkpKqqzGv93wpiAoy5wgNr9S3zMjI7CdIMnbAM
C1990
Sigma-Aldrich

CelLytic(TM) Express

Nacres: Express
Nhóm sản phẩm
MÃ SẢN PHẨM Kích cỡ gói Sẵn có Giá Số lượng
Request a Bulk Order Kíểm Tra Còn Hàng

Thuộc tính

Thuộc tính
Description CelLytic(TM) Express
form powder
Yêu cầu giấy phép Temp. Regu
Kiểm tra hết hạn No
Kiểm soát nhiệt độ -40
Hình thức vận chuyển DAS
Lĩnh vực sản phẩm Biology

Mô tả

Application

CelLytic Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris, and is immediately ready for affinity purification. CelLytic Express′ unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.

Features and Benefits

  • Efficient and non-denaturing in-culture protein extraction
  • Save time by eliminating cell harvest and clarification steps
  • Preserve biological activity with fewer manipulations
  • Compatible with affinity resins and magnetic beads
  • Ideal for Production Scale Extraction protocols

General description

CelLytic Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris and is immediately ready for affinity purification. CelLytic Express unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.

Preparation Note

CelLytic® Express is supplied ready-to-use. However, additional reagents may be used depending on the nature of the protein to be isolated. These include salts, protease inhibitors, and reducing agents such as dithiothreitol or 2-mercaptoethanol. The additional reagents should be added into the bacterial culture after dissolving the CelLytic Express, and not by adding them to the dry powder.

Storage and Stability

This product ships on wet ice and storage at –20°C is recommended. CelLytic Express, as supplied, is stable for at least two years when stored properly.

THÔNG TIN AN TOÀN

Signal Word

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Flash Point (C)

Target Organs

Risk Statement

Cảnh cáo nguy cơ bổ sung

TÀI LIỆU

Chứng nhận phân tích View Sample COA
Chứng nhận xuất xứ

Hỗ Trợ Kỹ Thuật

Đội ngũ các nhà khoa học của chúng tôi có kinh nghiệm trong tất cả các lĩnh vực nghiên cứu bao gồm Khoa học Đời sống, Khoa học Vật liệu, Tổng hợp Hóa học, Sắc ký, Phân tích và nhiều lĩnh vực khác.